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c caviae gpic  (ATCC)


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    ATCC c caviae gpic
    C Caviae Gpic, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c+caviae+gpic/Chlamydia+caviae/pmc04412952-60-14-17
    Average 93 stars, based on 6 article reviews
    c caviae gpic - by Bioz Stars, 2026-10
    93/100 stars

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    other:

    Article Title: Identification and characterization of Inc766, an inclusion membrane protein in Chlamydophila abortus-infected cells.
    Article Snippet: We have identified the gene product of locus 766 in the transmembrane head region (TMH/Inc-region) in the Chlamydophila abortus genome by using mass spectrometry and a monoclonal antibody that reacted with the inclusion membrane.. The identified protein at 32 kDa, termed Inc766, formed highly stable oligomers when solubilized in the absence of b-mercaptoethanol.. These oligomers were resistant to SDS, to heat denaturation and to 8 M urea, but very sensitive to b-mercaptoethanol, consistent with conformations resulting from protein–protein interactions stabilized through disulphide bonds.

    Infection:

    Article Title: Defining Species-Specific Immunodominant B Cell Epitopes for Molecular Serology of Chlamydia Species
    Article Snippet: Of the nine Chlamydia spp. ( 1 ), the strains C. abortus B577 (ATCC VR 656, DSMZ DSM 27654), C. pecorum E58 (ATCC VR 628), C. pneumoniae CDC/CWL-029 (ATCC VR-1310), and C. trachomatis D UW-3/CX (ATCC VR 885, DSMZ DSM 19411) were grown on buffalo green monkey kidney (BGMK) cells, and elementary bodies (EBs) were purified and stored at −80°C ( 67 ). .. Strains of the remaining five Chlamydia spp., i.e., C. psittaci 02DC15 (DSMZ DSM 27008), C. caviae GPIC (ATCC VR 813, DSMZ DSM 19441), C. felis 02DC26 (Collection FLI, Jena, Germany), C. muridarum MoPn/Nigg (ATCC VR 123, DSMZ DSM 28544), and C. suis 99DC3 (collection FLI), were propagated in developing chicken embryos, and infected yolk sacs were homogenized in sucrose phosphate glutamate (SPG) buffer with disposable tissue grinders and stored at −80°C ( 68 ). ..

    Article Title: Defining Species-Specific Immunodominant B Cell Epitopes for Molecular Serology of Chlamydia Species
    Article Snippet: Of the nine Chlamydia spp. (1), the strains C. abortus B577 (ATCC VR 656, DSMZ DSM 27654), C. pecorum E58 (ATCC VR 628), C. pneumoniae CDC/CWL-029 (ATCC VR-1310), and C. trachomatis D UW-3/CX (ATCC VR 885, DSMZ DSM 19411) were grown on buffalo green monkey kidney (BGMK) cells, and elementary bodies (EBs) were purified and stored at 80°C (67). .. Strains of the remaining five Chlamydia spp., i.e., C. psittaci 02DC15 (DSMZ DSM 27008), C. caviae GPIC (ATCC VR 813, DSMZ DSM 19441), C. felis 02DC26 (Collection FLI, Jena, Germany), C. muridarum MoPn/Nigg (ATCC VR 123, DSMZ DSM 28544), and C. suis 99DC3 (collection FLI), were propagated in developing chicken embryos, and infected yolk sacs were homogenized in sucrose phosphate glutamate (SPG) buffer with disposable tissue grinders and stored at 80°C (68). ..



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    Assay of the GAG dependency of binding by C. <t>caviae</t> OmcB and C. trachomatis OmcB variants. Schematic representation of the adhesion of various yeast transformants to HEp-2 cells. Yeast cells expressing Aga2, Aga2–OmcB- C.pn ., Aga2–OmcB- C.ca. <t>GPIC,</t> Aga2–OmcB- C.tr. L1, Aga2–OmcB- C.tr . E, Aga2–OmcB- C.tr . L1 P 66 L or Aga2–OmcB- C.tr . E L 66 P were pre-incubated with 500 μg ml −1 soluble heparin prior to the adhesion assay. ( n = 1000 HEp-2 cells, No. experiments = 4, P < 0.0001). w/o, without; C.pn ., C. pneumoniae ; C.ca. , C. caviae ; C.tr. , C. trachomatis .
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    c caviae gpic t  (ATCC)
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    Assay of the GAG dependency of binding by C. <t>caviae</t> OmcB and C. trachomatis OmcB variants. Schematic representation of the adhesion of various yeast transformants to HEp-2 cells. Yeast cells expressing Aga2, Aga2–OmcB- C.pn ., Aga2–OmcB- C.ca. <t>GPIC,</t> Aga2–OmcB- C.tr. L1, Aga2–OmcB- C.tr . E, Aga2–OmcB- C.tr . L1 P 66 L or Aga2–OmcB- C.tr . E L 66 P were pre-incubated with 500 μg ml −1 soluble heparin prior to the adhesion assay. ( n = 1000 HEp-2 cells, No. experiments = 4, P < 0.0001). w/o, without; C.pn ., C. pneumoniae ; C.ca. , C. caviae ; C.tr. , C. trachomatis .
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    Assay of the GAG dependency of binding by C. caviae OmcB and C. trachomatis OmcB variants. Schematic representation of the adhesion of various yeast transformants to HEp-2 cells. Yeast cells expressing Aga2, Aga2–OmcB- C.pn ., Aga2–OmcB- C.ca. GPIC, Aga2–OmcB- C.tr. L1, Aga2–OmcB- C.tr . E, Aga2–OmcB- C.tr . L1 P 66 L or Aga2–OmcB- C.tr . E L 66 P were pre-incubated with 500 μg ml −1 soluble heparin prior to the adhesion assay. ( n = 1000 HEp-2 cells, No. experiments = 4, P < 0.0001). w/o, without; C.pn ., C. pneumoniae ; C.ca. , C. caviae ; C.tr. , C. trachomatis .

    Journal: Molecular Microbiology

    Article Title: The Chlamydia outer membrane protein OmcB is required for adhesion and exhibits biovar-specific differences in glycosaminoglycan binding

    doi: 10.1111/j.1365-2958.2007.06050.x

    Figure Lengend Snippet: Assay of the GAG dependency of binding by C. caviae OmcB and C. trachomatis OmcB variants. Schematic representation of the adhesion of various yeast transformants to HEp-2 cells. Yeast cells expressing Aga2, Aga2–OmcB- C.pn ., Aga2–OmcB- C.ca. GPIC, Aga2–OmcB- C.tr. L1, Aga2–OmcB- C.tr . E, Aga2–OmcB- C.tr . L1 P 66 L or Aga2–OmcB- C.tr . E L 66 P were pre-incubated with 500 μg ml −1 soluble heparin prior to the adhesion assay. ( n = 1000 HEp-2 cells, No. experiments = 4, P < 0.0001). w/o, without; C.pn ., C. pneumoniae ; C.ca. , C. caviae ; C.tr. , C. trachomatis .

    Article Snippet: The full-length omcB coding sequence or omcB variants were amplified by PCR from genomic C. pneumoniae GiD, C. trachomatis serovar LGV L1/440/Bu, C. trachomatis serovar E DK-20 or C. caviae GPIC DNA, and either cloned directly into the EcoRI+NotI-digested pYD1 vector (Invitrogen) or cloned into the BglII and EcoRI sites of the pAC-2 vector after attachment of a 5′ sequence encoding a His 6 -Tag.

    Techniques: Binding Assay, Expressing, Incubation, Cell Adhesion Assay